1T48
Allosteric Inhibition of Protein Tyrosine Phosphatase 1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-04-17 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.941, 88.941, 104.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.2132 |
Rwork | 0.211 |
R-free | 0.25451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pty |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.780 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 24798 | |
Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |