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1T3Z

Formyl-CoA Tranferase mutant Asp169 to Ser

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsMAX II BEAMLINE I711
Synchrotron siteMAX II
BeamlineI711
Temperature [K]110
Detector technologyCCD
Collection date2004-02-08
DetectorMARRESEARCH
Wavelength(s)1.087
Spacegroup nameI 4
Unit cell lengths151.825, 151.825, 100.227
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.300
R-factor0.17893
Rwork0.177
R-free0.21576
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)1p5r
RMSD bond length0.013
RMSD bond angle1.476
Data reduction softwareMOSFLM
Data scaling softwareCCP4 ((SCALA))
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.2902.420
High resolution limit [Å]2.3002.300
Number of reflections50343
<I/σ(I)>173.6
Completeness [%]99.699.6
Redundancy4.34.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293PEG 4000, Hepes, Mg chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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