1T3S
Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9333 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.066, 163.841, 34.761 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.650 - 2.300 |
R-factor | 0.26222 |
Rwork | 0.261 |
R-free | 0.27728 |
Structure solution method | MAD |
Starting model (for MR) | model built from earlier MAD data |
RMSD bond length | 0.008 |
RMSD bond angle | 1.536 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.048 | 0.310 |
Number of reflections | 18271 | |
<I/σ(I)> | 15.4 | 4.3 |
Completeness [%] | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | PEG 20K, BICINE, SODIUM CHLORIDE, BETA-MERCAPTOETHANOL, MPD, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 292K |