1T3D
Crystal structure of Serine Acetyltransferase from E.coli at 2.2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.980, 121.980, 127.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 2.200 |
| R-factor | 0.175 |
| Rwork | 0.151 |
| R-free | 0.17700 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.642 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.200 |
| Number of reflections | 51271 | |
| <I/σ(I)> | 12.1 | 5 |
| Completeness [%] | 91.5 | 77 |
| Redundancy | 5.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | MES, MPD, sodium thiocyanate, cysteine, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
