1T2X
Glactose oxidase C383S mutant identified by directed evolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.376, 88.995, 85.800 |
| Unit cell angles | 90.00, 117.49, 90.00 |
Refinement procedure
| Resolution | 61.980 - 2.300 |
| R-factor | 0.2278 |
| Rwork | 0.218 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gog |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.946 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.980 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | 0.387 |
| Number of reflections | 28708 | |
| <I/σ(I)> | 12.9 | 2.2 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 280 | Ammonium sulphate, PEG 4000, Calcium Acetate, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
