1T2K
Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 186.380, 65.210, 83.850 |
| 格子定数 [度] | 90.00, 93.44, 90.00 |
精密化法
| 残基 | 29.980 - 3.000 |
| R因子 | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2IRF and 2DGC |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 3.190 |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| 独立反射数 | 20151 | |
| <I/σ(I)> | 19.4 | 5.7 |
| 完全性 [%] | 94 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 100 mM NaCacodylate, pH 6.5, 12.5% (w/v) PEG 6000, 400 mM NH4OAc, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | NaCacodylate | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NH4OAc | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 6000 | ||
| 6 | 1 | 2 | NaCacodylate | ||
| 7 | 1 | 2 | H2O |
