1T2H
Y81W mutant of RNase Sa from Streptomyces aureofaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.206, 64.309, 77.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.000 |
| R-factor | 0.14631 |
| Rwork | 0.145 |
| R-free | 0.16593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.478 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.010 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.047 | 0.165 |
| Number of reflections | 103514 | |
| <I/σ(I)> | 34.5 | 10.2 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 6.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | ammonium sulfate, disodium phosphate, monosodium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
