1T20

Structural basis for degenerate recognition of HIV peptide variants by cytotoxic lymphocyte, variant SL9-6I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-05
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	56.460, 80.080, 57.960
Unit cell angles	90.00, 116.38, 90.00

Refinement procedure

Resolution	11.930 - 2.200
R-factor	0.197
Rwork	0.197
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hla
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Number of reflections	21603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG, MES, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K