1T1C
Late intermediate IL3 from time-resolved crystallography of the E46Q mutant of PYP
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 288 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.917, 66.917, 41.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1294 |
| Rwork | 0.129 |
| R-free | 0.13860 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data scaling software | LaueView |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 13957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
