1T0J
Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.756, 45.330, 60.652 |
| Unit cell angles | 96.81, 102.46, 98.53 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20188 |
| Rwork | 0.200 |
| R-free | 0.24154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1toh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.616 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 24890 | |
| <I/σ(I)> | 11.65 | 2.06 |
| Completeness [%] | 97.3 | 96.1 |
| Redundancy | 2.49 | 2.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Tris-Cl, PEG 4000, NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
