1T0I
YLR011wp, a Saccharomyces cerevisiae NA(D)PH-dependent FMN reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979678 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.801, 109.957, 49.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.20175 |
Rwork | 0.199 |
R-free | 0.25436 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.743 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 27164 | |
<I/σ(I)> | 21.9 | |
Completeness [%] | 96.9 | 95.5 |
Redundancy | 4.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 10% PEG 3000, 30% PEG 400, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |