1T0F
Crystal Structure of the TnsA/TnsC(504-555) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.032, 70.112, 89.681 |
| Unit cell angles | 90.00, 123.61, 90.00 |
Refinement procedure
| Resolution | 42.410 - 1.850 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f1z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.078 | 0.386 |
| Number of reflections | 66363 | |
| <I/σ(I)> | 9.3 | 2.37 |
| Completeness [%] | 97.9 | 99.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | malonate, lithium sulfate, 2,4 methylpentanediol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
