1T0B
Structure of ThuA-like protein from Bacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-06-23 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.28136 |
Spacegroup name | P 1 |
Unit cell lengths | 71.542, 74.603, 123.518 |
Unit cell angles | 90.84, 97.78, 118.69 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.14484 |
Rwork | 0.145 |
R-free | 0.17536 |
Structure solution method | Se-SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 1.951 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 207543 |
Completeness [%] | 85.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | VAPOR DIFFUSION, HANGING DROP, temperature 273K |