1T06
1.9 A Crystal Structure of a Protein of Unknown Function from Bacillus cereus ATCC 14579
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-30 |
Detector | SBC-2 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.959, 89.112, 121.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 1.900 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24100 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.110 | 0.437 |
Number of reflections | 72674 | |
<I/σ(I)> | 18.18 | 2.12 |
Completeness [%] | 95.9 | 77 |
Redundancy | 6.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1.2M NH4SO4, 20% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |