1SZU

The structure of gamma-aminobutyrate aminotransferase mutant: V241A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-18
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	108.200, 108.200, 301.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.520
R-factor	0.156
Rwork	0.154
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.440
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.520	2.520
Number of reflections	68806
Completeness [%]	98.2	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	HEPES, ammonium sulfate, PLP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K