1SZK
The structure of gamma-aminobutyrate aminotransferase mutant: E211S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-08-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 108.260, 108.260, 300.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.520 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sf2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 69873 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 97.5 | 97.5 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | HEPES, Ammonium Sulfate, PLP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |