1SZK
The structure of gamma-aminobutyrate aminotransferase mutant: E211S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.260, 108.260, 300.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.520 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sf2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 69873 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 97.5 | 97.5 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | HEPES, Ammonium Sulfate, PLP, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
