1SZG
A198G:L230A flavocytochrome b2 with sulfite bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8700 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 163.966, 163.966, 112.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.000 - 2.700 |
| R-factor | 0.22495 |
| Rwork | 0.223 |
| R-free | 0.26567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb id 1LTD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.119 | 0.600 |
| Number of reflections | 43777 | |
| <I/σ(I)> | 11.44 | 2.66 |
| Completeness [%] | 91.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES, 12-15% PEG4000, 18-21% glycerol, 50 mM sodium sulfite, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
