1SZ9
The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-09 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.730, 67.670, 135.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.25200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | ORIGINAL MODEL FROM MAD EXPERIMENT WAS USED FOR FURTHER REFINEMENT. R-FREE SET WAS KEPT CONSISTEND |
RMSD bond length | 0.009 |
RMSD bond angle | 1.211 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 29988 | |
<I/σ(I)> | 13.5 | 4.5 |
Completeness [%] | 98.0 | 96.7 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 25 % (v/v) ethylene glycol, 5 % (v/v) PEG 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 7.50 |