1SZ8

Crystal Structure of an Acidic Phospholipase A2 from Naja Naja Sagittifera at 1.5 A resolution

Replaces:  1LFF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	200
Detector technology	CCD
Collection date	2001-09-13
Detector	MARRESEARCH
Spacegroup name	P 41
Unit cell lengths	42.017, 42.017, 64.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.192
Rwork	0.191
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1psh
RMSD bond length	0.009
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	17571
<I/σ(I)>	26.63	3
Completeness [%]	97.5	91.6
Redundancy	13.6	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Ammonium Sulphate, CaCl2, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K