1SZ1
Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 218.403, 218.403, 170.263 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.720 - 6.210 |
R-factor | 0.36131 |
Rwork | 0.360 |
R-free | 0.37962 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r89 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.378 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 6.210 | 6.210 |
Rmerge | 0.102 | |
Number of reflections | 10111 | |
<I/σ(I)> | 12.2 | 1.5 |
Completeness [%] | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | (NH4)2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | H2O | ||
3 | 1 | 2 | (NH4)2SO4 | ||
4 | 1 | 2 | H2O |