1SZ0
N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.100, 86.500, 84.800 |
| Unit cell angles | 90.00, 109.57, 90.00 |
Refinement procedure
| Resolution | 28.800 - 2.100 |
| R-factor | 0.2381 |
| Rwork | 0.238 |
| R-free | 0.28700 |
| Structure solution method | RIGID BODY REFINEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 45601 | |
| Completeness [%] | 81.2 | 28.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.35 | 292 | 25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
