1SYS

Crystal structure of HLA, B*4403, and peptide EEPTVIKKY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.823, 81.985, 110.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.400
Rwork	0.241
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.400
Number of reflections	18236

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	295	PEG 4000, 0.2M Ammonium Acetate, 0.2M Sodium Citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K