1SYO
N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-14 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.400, 86.400, 86.900 |
Unit cell angles | 90.00, 108.80, 90.00 |
Refinement procedure
Resolution | 29.960 - 2.200 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q25 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.064 | 0.301 |
Number of reflections | 38820 | |
Completeness [%] | 88.8 | 55.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.35 | 292 | 25% PEG 4000, 0.1M sodium cacodylate (pH 6.35), 150mM NaCl, 50 mM imidazole (pH 6.7), 10 mM manganese chloride, 5mM beta-glycerophosphate, 10 mM mannose 6-phosphate, vapor diffusion, sitting drop, temperature 292K |