1SXZ
Reduced bovine superoxide dismutase at pH 5.0 complexed with azide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11-15 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.600, 197.500, 50.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.050 |
R-factor | 0.166 * |
Rwork | 0.166 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sxc |
RMSD bond length | 0.018 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.074 | 0.183 |
Total number of observations | 213345 * | |
Number of reflections | 30955 * | |
<I/σ(I)> | 12.6 | 8.07 |
Completeness [%] | 87.8 | 85.5 |
Redundancy | 5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | free interface diffusion * | 5 | 22 * | 20 % PEG6K, 20MM HEPES PH 5.0, SODIUM DITHIONITE (EXCESS), NITROGEN ATMOSPHERE, excess nitrogen atmosphere |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG6000 | 20 (%) | |
2 | 1 | 1 | 50 (mM) | ||
3 | 1 | 2 | protein | 7 (mg/ml) |