1SXY
1.07 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.142, 42.776, 52.979 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.070 |
| R-factor | 0.15069 |
| Rwork | 0.150 |
| R-free | 0.17199 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2u |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 1.110 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.056 | 0.201 |
| Number of reflections | 67346 | |
| <I/σ(I)> | 24.2 | 9.1 |
| Completeness [%] | 97.6 | 96.8 |
| Redundancy | 5.37 | 5.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Phosphate 3.2 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
