1SXW
1.05 A Crystal Structure of D30A Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Nitric Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.358, 42.839, 53.077 |
| Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
| Resolution | 16.240 - 1.050 |
| R-factor | 0.15354 |
| Rwork | 0.152 |
| R-free | 0.17857 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1koi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.900 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.059 | 0.259 |
| Number of reflections | 71942 | |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 97.9 | 99.4 |
| Redundancy | 2.98 | 2.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium Phosphate 3.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
