1SXU
1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.135, 42.541, 52.995 |
| Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
| Resolution | 20.400 - 1.400 |
| R-factor | 0.14264 |
| Rwork | 0.141 |
| R-free | 0.17534 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ikj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.816 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.400 | 1.410 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.034 | 0.070 |
| Number of reflections | 33304 | |
| <I/σ(I)> | 20.3 | 7.7 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 2.53 | 1.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
