1SXT

STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC

Experimental procedure

Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1995-06
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	75.590, 75.590, 175.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.000 - 2.700
R-factor	0.206
Rwork	0.206
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.650
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000 *	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.056 *	0.227 *
Number of reflections	15899
Completeness [%]	91.8
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.25	18 *	THE PROTEIN WAS PRE-INCUBATED WITH 0.1 MM ZNSO4. CRYSTALS WAS GROWN USING THE HANGING DROP METHOD FROM 15% PEG 6000, 0.3 M NH4SO4 AND 0.1 M MES BUFFER AT PH 6.25, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop		0.05 (mM)
3	1	drop	PEG6000	7.5 (%(w/v))
4	1	drop	ammonium sulfate	0.15 (M)
5	1	drop	MES	0.05 (M)
6	1	reservoir	PEG6000	15 (%(w/v))
7	1	reservoir	ammonium sulfate	0.3 (M)
8	1	reservoir	MES	0.1 (M)