1SXK
Crystal Structure of a complex formed between phospholipase A2 and a non-specific anti-inflammatory amino salicylic acid at 1.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2003-09-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8030 |
Spacegroup name | P 43 |
Unit cell lengths | 52.255, 52.255, 47.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.470 - 1.210 |
R-factor | 0.17388 |
Rwork | 0.174 |
R-free | 0.18845 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fb2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.646 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 38466 | |
<I/σ(I)> | 10.1 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 297 | 0.2M ammonium sulphate, 50% PEG, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |