1SXI
Structure of apo transcription regulator B. megaterium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.240, 106.220, 157.740 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 74.580 - 3.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sxh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.580 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 66347 | |
| Completeness [%] | 98.0 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8000, magnesium chloride, tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
