1SX6
Crystal structure of human Glycolipid Transfer protein in lactosylceramide-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-19 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.599, 49.073, 68.538 |
Unit cell angles | 90.00, 122.52, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.19479 |
Rwork | 0.192 |
R-free | 0.24429 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.240 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.117 | 0.490 |
Number of reflections | 16786 | |
<I/σ(I)> | 15 | 1.7 |
Completeness [%] | 99.6 | |
Redundancy | 4.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | PEG 8000, potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |