1SX5
K38A EcoRV bound to cleaved DNA and Mn2+: P1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.708, 48.627, 63.544 |
| Unit cell angles | 96.68, 108.94, 107.33 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.510 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.327 |
| Number of reflections | 74930 | |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 93.0 | 92.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 315 | PEG 4000, 0.1M HEPES, 0.15M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 315K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 2 | HEPES | ||
| 3 | 1 | 3 | NaCl |
