1SWU
STREPTAVIDIN MUTANT Y43F
Experimental procedure
Experimental method | MONOCHROMATIC |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-18 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.200, 84.900, 46.400 |
Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.140 |
R-factor | 0.125 |
R-free | 0.15700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swa |
RMSD bond length | 0.015 |
RMSD bond angle | 0.035 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.200 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.056 | 0.144 * |
Total number of observations | 627749 * | |
Number of reflections | 149978 | |
<I/σ(I)> | 13.6 | 5.8 |
Completeness [%] | 92.0 | 81 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.5 | pH 4.5 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
2 | 1 | reservoir | 50 (%) |