1SWS

CORE-STREPTAVIDIN MUTANT D128A AT PH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-11-13
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	48.000, 66.300, 82.300
Unit cell angles	90.00, 97.30, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.167 *
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swc
RMSD bond length	0.006
RMSD bond angle	0.024
Phasing software	SHELXS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.030
Total number of observations	56045 *
Number of reflections	30500
<I/σ(I)>	15	2
Completeness [%]	85.0	46
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5		50% MPD (2-METHYL-2,4-PENTANEDIOLE), PH 4.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	MPD	50 (%)