1SWS
CORE-STREPTAVIDIN MUTANT D128A AT PH 4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11-13 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.000, 66.300, 82.300 |
Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.167 * |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swc |
RMSD bond length | 0.006 |
RMSD bond angle | 0.024 |
Phasing software | SHELXS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.030 | |
Total number of observations | 56045 * | |
Number of reflections | 30500 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 85.0 | 46 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.5 | 50% MPD (2-METHYL-2,4-PENTANEDIOLE), PH 4.5. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | MPD | 50 (%) |