1SWP
CORE-STREPTAVIDIN MUTANT W120F IN COMPLEX WITH BIOTIN AT PH 7.5
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1996-11-13 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.800, 100.000, 51.800 |
Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.251 |
R-free | 0.27800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swe |
RMSD bond length | 0.005 |
RMSD bond angle | 0.019 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.300 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.350 |
Total number of observations | 62223 * | |
Number of reflections | 24596 | |
<I/σ(I)> | 6.9 | 1.5 |
Completeness [%] | 90.0 | 75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | drop | biotin | 2.5 molar excess | |
3 | 1 | reservoir | PEG4000 | 12-20 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) |