1SWL

CORE-STREPTAVIDIN MUTANT W108F AT PH 7.0

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1995-09-18
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	58.600, 87.200, 47.200
Unit cell angles	90.00, 98.90, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.183 *
R-free	0.24900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.060	0.144
Total number of observations	40162 *
Number of reflections	33423
<I/σ(I)>	4.7	1.82
Completeness [%]	85.0	59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	PEG1000	22-24 (%)
3	1	reservoir	Tris-HCl	0.1 (M)