1SWK
CORE-STREPTAVIDIN MUTANT W79F IN COMPLEX WITH BIOTIN AT PH 4.5
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-30 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.300, 64.600, 76.000 |
Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.159 * |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.025 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.100 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.290 |
Total number of observations | 38347 * | |
Number of reflections | 38017 | |
<I/σ(I)> | 6.9 | 2.7 |
Completeness [%] | 97.0 | 87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.5 | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | reservoir | MPD | 48 (%) | |
3 | 1 | drop | biotin | 2.5 molar excess |