1SWI
GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-08 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.210, 46.410, 52.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.600 |
R-factor | 0.187 |
Rwork | 0.187 |
RMSD bond length | 0.019 |
RMSD bond angle | 3.150 |
Data reduction software | R-AXIS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 * |
Rmerge | 0.097 |
Number of reflections | 2618 |
Completeness [%] | 86.0 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | peptide | 20 (mg/ml) | |
2 | 1 | drop | sodium citrate | 1.0 (M) | |
3 | 1 | drop | HEPES | 100 (mM) | |
4 | 1 | reservoir | benzene | 0.02 ml to 1 ml of reservoir buffer |