1SWH

CORE-STREPTAVIDIN MUTANT W79F AT PH 4.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-03-16
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	58.300, 86.700, 46.900
Unit cell angles	90.00, 98.80, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.157 *
R-free	0.23700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pts A SECOND DIMER WAS CONSTRUCTED USING THE TWO-FOLD AXIS
RMSD bond length	0.006
RMSD bond angle	0.025
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.040	0.118
Total number of observations	54975 *
Number of reflections	31526
<I/σ(I)>	10.2	3.5
Completeness [%]	81.0	47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	MPD	46 (%)