1SWF

CIRCULAR PERMUTED STREPTAVIDIN E51/A46

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-06-24
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	60.296, 78.596, 93.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.19
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SWA (EXCLUDING RESIDUES 46 - 51 IN ALL SUBUNITS)
RMSD bond length	0.006
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.380
Total number of observations	93577 *
Number of reflections	28795
<I/σ(I)>	5	2
Completeness [%]	94.0	82.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		PROTEIN WAS CRYSTALLIZED FROM 52% MPD (PH 4.5).

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	MPD	52 (%)