1SWE
APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN AT PH 4.5
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1996-02-13 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.780, 100.110, 52.010 |
Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.060 |
R-factor | 0.161 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swa |
RMSD bond length | 0.006 |
RMSD bond angle | 0.025 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | SHELXL-96 |
Refinement software | SHELXL-96 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 | 0.192 |
Number of reflections | 24738 | |
<I/σ(I)> | 9 | 3.4 |
Completeness [%] | 83.0 | 42 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | PROTEIN-BIOTIN COMPLEX WAS CO-CRYSTALLIZED FROM 50% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5) WITH 2.5M EXCESS OF BIOTIN |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-30 (mg/ml) | |
2 | 1 | drop | MPD | 100 (%) | |
3 | 1 | drop | biotin | 2.5 (M) | excess |
4 | 1 | reservoir | MPD | 50 (%) |