1SWE

APO-CORE-STREPTAVIDIN IN COMPLEX WITH BIOTIN AT PH 4.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-02-13
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	52.780, 100.110, 52.010
Unit cell angles	90.00, 112.60, 90.00

Refinement procedure

Resolution	8.000 - 2.060
R-factor	0.161
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.006
RMSD bond angle	0.025
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.066	0.192
Number of reflections	24738
<I/σ(I)>	9	3.4
Completeness [%]	83.0	42
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		PROTEIN-BIOTIN COMPLEX WAS CO-CRYSTALLIZED FROM 50% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5) WITH 2.5M EXCESS OF BIOTIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	MPD	100 (%)
3	1	drop	biotin	2.5 (M)	excess
4	1	reservoir	MPD	50 (%)