1SWC

APO-CORE-STREPTAVIDIN AT PH 4.5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-01-04
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	47.800, 65.900, 82.100
Unit cell angles	90.00, 97.40, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.159
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swa
RMSD bond length	0.007
RMSD bond angle	0.027
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	SHELXL-96
Refinement software	SHELXL-96

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.460	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.126
Number of reflections	48224
<I/σ(I)>	12	3.3
Completeness [%]	79.0	48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		PROTEIN WAS CRYSTALLIZED FROM 60% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5), SOAKING IN 20 MM SODIUM ACETATE BUFFER PH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	reservoir	MPD	60 (%)