1SWC
APO-CORE-STREPTAVIDIN AT PH 4.5
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1994-01-04 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.800, 65.900, 82.100 |
Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.159 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.027 |
Data reduction software | PROCESS |
Data scaling software | PROCESS |
Phasing software | SHELXL-96 |
Refinement software | SHELXL-96 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.460 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.126 |
Number of reflections | 48224 | |
<I/σ(I)> | 12 | 3.3 |
Completeness [%] | 79.0 | 48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | PROTEIN WAS CRYSTALLIZED FROM 60% MPD (2-METHYL-PENTANE-2,4-DIOLE, PH 4.5), SOAKING IN 20 MM SODIUM ACETATE BUFFER PH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-30 (mg/ml) | |
2 | 1 | reservoir | MPD | 60 (%) |