1SWA
APO-CORE-STREPTAVIDIN AT PH 4.5
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-11 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.500, 87.100, 47.000 |
| Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.147 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NOT PUBLISHED YET STREPTAVIDIN MUTANT STRUCTURE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.025 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | SHELXL-96 |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.540 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.170 |
| Number of reflections | 26574 | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 79.0 | 28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | PROTEIN WAS CRYSTALLIZED FROM 47% MPD (PH 4.5) |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 47 (%) |
