1SW6

S. CEREVISIAE SWI6 ANKYRIN-REPEAT FRAGMENT

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X31
Temperature [K]	100
Wavelength(s)	0.9, 0.9795, 0.9799
Spacegroup name	P 21 21 21
Unit cell lengths	76.850, 91.730, 90.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.226 *
Rwork	0.222
R-free	0.27900 *
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	2.100 *
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.500
Rmerge	0.119
Number of reflections	8625
<I/σ(I)>	12
Completeness [%]	99.6
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	16 *	pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG3350	24-27 (%)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		300 (mM)