1SVW
Crystal Structure of YsxC complexed with GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.586, 70.599, 72.583 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| Rwork | 0.306 |
| R-free | 0.33500 |
| Structure solution method | MIR |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.057 | 0.112 |
| Number of reflections | 10097 | |
| <I/σ(I)> | 16.9 | 9.5 |
| Completeness [%] | 97.4 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | PEG 8000, Hepes, Ethylene glycol, GMPPNP, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
