1SVT
Crystal structure of GroEL14-GroES7-(ADP-AlFx)7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 255.546, 266.855, 187.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.386 - 2.808 |
R-factor | 0.248 |
Rwork | 0.247 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 2.039 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (refmac_5.1.19) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 231127 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG3000, cacodylic acid, potassium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG3000, cacodylic acid, potassium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |