1SVN
SAVINASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | FILM |
| Collection date | 1991 |
| Detector | CEA FILM |
| Wavelength(s) | 1.009, 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.470, 64.240, 42.890 |
| Unit cell angles | 90.00, 118.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.174 * |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.051 |
| Data reduction software | MOSFLM |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 2.500 * |
| High resolution limit [Å] | 1.400 * |
| Rmerge | 0.083 |
| Number of reflections | 29065 * |
| Completeness [%] | 77.0 * |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 4 (%) | |
| 3 | 1 | drop | 0.33 (M) | ||
| 4 | 1 | drop | 1.5 (mM) | ||
| 5 | 1 | drop | citrate | 18 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 7 | 1 | reservoir | 1 (M) | ||
| 8 | 1 | reservoir | 5 (mM) | ||
| 9 | 1 | reservoir | citrate | 50 (mM) |
