1SVC
NFKB P50 HOMODIMER BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 113 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 137.000, 137.000, 57.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.600 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28600 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.800 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.045 * |
| Number of reflections | 15658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | HEPES | ||
| 5 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein dimer | 0.18 (mM) | |
| 2 | 1 | drop | DNA duplex | 0.22 (mM) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | spermine | 2 (mM) | |
| 9 | 1 | reservoir | PEG3000 | 1-4 (%) |
