1SV0

Crystal Structure Of Yan-SAM/Mae-SAM Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	67.651, 70.327, 88.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.810 - 2.070
R-factor	0.232
Rwork	0.232
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.070	2.070
Number of reflections	26004
<I/σ(I)>	28.63	5.8
Completeness [%]	99.9	100
Redundancy	14	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K