1SUW
Crystal structure of a NAD kinase from Archaeoglobus fulgidus in complex with its substrate and product: Insights into the catalysis of NAD kinase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9796, 0.9798, 0.9600 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 122.141, 122.141, 198.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.25220 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.743 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.560 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 62750 | |
Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM Hepes, 2M NaCl, 10% PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |